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- Advantec MCE, Sterile - MCE Membranes
Descripción general
Advantec MCE, Sterile - MCE Membranes
Preguntas frecuentes
The pores of microporous membrane filters act as small capillaries. When hydrophilic membranes come into contact with water, capillary action associated with surface tension forces causes the water to spontaneously enter and fill the pores. In this manner, the membranes are easily wetted and allow the bulk flow of water through the pores. Once wetted, hydrophilic membranes will not allow the bulk flow of air or other gasses, unless they are applied at pressures greater than the membrane’s bubble point.
Hydrophilic membrane filters are typically used with water and aqueous solutions. They can also be used with compatible non-aqueous fluids. Hydrophilic membrane filters are typically not used for air, gas or vent filtration since the filters would block flow if inadvertently wetted, by condensation for example.
When hydrophobic membranes come into contact with water, surface tension forces act to repel the water from the pores. Water will not enter the pores and the membranes will act as a barrier to water flow, unless the water is applied at pressures greater than the membrane’s water entry pressure. Low surface tension fluids, such as alcohols, can spontaneously enter and fill the pores of hydrophobic membranes. Once all the air in the pores is displaced, there are no longer any surface tension forces and water can easily enter the pores, displace the low surface tension fluid, and pass through the membrane. The membrane will then allow bulk flow of water for as long as the pore remain water filled. If the membrane is allowed to dry (i.e. air enters the pores), then it must be pre-wet with a low surface tension fluid again prior to use with water.
Hydrophobic membrane filters are typically used with compatible non-aqueous fluids. They are also commonly used as air, gas, or vent filters. Hydrophobic membrane filters are sometimes used with water or aqueous solutions; and, in these applications, they must first be prewet with a low surface tension, water miscible fluid prior to use.
MCE membranes feature fast flow rates, a high protein binding capacity, and great thermal stability, making them a staple for many environmental and biological laboratories. Furthermore, they are available as presterilized, individually wrapped membranes, and can include a gridded pattern for quantifying microbial growth.
The maximum operating temperatures for Sterlitech filter membranes are listed below.
- Sterlitech Silver Metal - 427°C
- Sterlitech Ceramic - 350°C
- Sterlitech Polycarbonate Track Etch - 140°C
- Sterlitech Polyester - 140°C
- Sterlitech Nitrocellulose (MCE) - 130°C
- Sterlitech Nylon - 180°C
- Sterlitech Polyethersulfone (PES) - *130°C
- Sterlitech Polypropylene - 82°C
- Sterlitech Cellulose Acetate - 135°C
- Sterlitech PTFE (Laminated) - 130°C
- Sterlitech PTFE (Unlaminated) - 260°C
- Sterlitech PVC Data not available
*5.0um and 8.0um - max temp is 180°C
Cellulose acetate (CA) and nitrocellulose (MCE) membranes will last 2 1/2 to 3 years before reverting to their natural hydrophobic states. Nylon, on the other hand, is naturally hydrophilic so it does not have a shelf life.
An easy test for older membranes is to perform a "wetting out" test. Dipping a small portion of the membrane into water (hydrophilic membranes) or an alcohol (hydrophobic membranes) works well. If the membrane absorbs the material, or "wets out", it has not gone past the shelf life.
Ideally, nitrocellulose (NC) membranes would be made entirely of nitrocellulose polymer. However, almost all commercially available filtration grade membrane filters labeled as “nitrocellulose” are actually composed of membranes made with a mixture of nitrocellulose and cellulose acetate polymers. This is because pure nitrocellulose membrane filters are very difficult to manufacture with acceptable characteristics.
In an effort to provide clarity to consumers, some manufactures refer to membranes containing a mixture of nitrocellulose and cellulose acetate polymers as mixed cellulose esters (MCE) membranes. In almost all filtration applications, mixed cellulose esters (MCE) membrane filters are equivalent to nitrocellulose (NC) membrane filters.
We have a Chemical Compatibility Chart that you can use for reference.
Nominal pore size ratings are provided as a general indication of filter retention. It is understood that some quantity of particles greater than, and equal to, the nominal pore size ratings will pass through the filters into the filtrate. Some manufactures may associate nominal pore size ratings with percentage filtration efficiencies. Nominal pore size ratings vary from manufacturer to manufacturer and, consequently, are not necessarily equivalent. Filters from different manufactures with similar nominal pore size ratings may not actually exhibit similar retention characteristics.
Absolute Tamaño de poro ratings are typically based on retention studies performed using challenge suspensions of standard microorganism cultures or particles of known size. Absolute Tamaño de poro ratings represent the size of the smallest microorganisms or particles completely retained during these studies. Absolute Tamaño de poro ratings are almost always correlated to bubble point Especificaciones that are used for quality control during membrane manufacturing. For the most part, absolute Tamaño de poro ratings, especially those based on microbial retention, are comparable from manufacturer to manufacturer. There is more uncertainty for absolute Tamaño de poro ratings based on particle retention studies, especially for Tamaño de poro ratings <0.2µm, since there are no standard methods for these studies.
Regardless of pore size ratings, it is important to understand that application conditions do influence particle retention. Even filters with absolute pore size ratings can be operated in conditions that will allow unexpectedly sized particles to pass.
The Tamaño de poro refers to the diameter of the individual pores in a Filtro de membrana. Tamaño de poro is typically specified in micrometers (µm). Most membranes and filter media actually contain a distribution of pore sizes. Nominal Tamaño de poro ratings typically refer to the predominant Tamaño de poro of a filtration media; pores larger and smaller than the nominal rating may be present. Absolute Tamaño de poro ratings typically refer to the largest Tamaño de poro of a membrane and it is expected that all pores will be equal to or smaller than the absolute rating.
For the polycarbonate track-etch (PCTE) and polyester track-etch (PETE) Filtros de membrana, porosity is the percent of the total surface area occupied by the pores; it typically ranges from <1% to 16%. For the other Filtros de membrana, porosity is the percent of the total volume occupied by the pores; it typically ranges from 40 to 80%.
| Especificación |
0.2 µm · 47 mm · pack 100 · A020G047A
|
0.2 µm · 47 mm · pack 100 · A020H047K
|
0.2 µm · 47 mm · pack 100 · A020H047A
|
0.2 µm · 47 mm · pack 100 · A020G047J
|
0.45 µm · 47 mm · pack 100 · A045F047A
|
0.45 µm · 47 mm · pack 100 · A045H047A
|
0.45 µm · 47 mm · pack 100 · A045G047A
|
0.45 µm · 47 mm · pack 100 · A045M047A
|
0.45 µm · 47 mm · pack 100 · A045R047A
|
0.45 µm · 47 mm · pack 100 · A045H047K
|
0.45 µm · 47 mm · pack 100 · A045G047J
|
0.45 µm · 47 mm · pack 100 · A045W047A
|
0.45 µm · 47 mm · pack 1000 · A045F047W
|
0.45 µm · 47 mm · pack 1000 · A045H047W
|
0.45 µm · 47 mm · pack 1000 · A045R047W
|
0.45 µm · 47 mm · pack 200
|
0.45 µm · 85 mm · pack 100
|
0.65 µm · 47 mm · pack 100
|
0.8 µm · 47 mm · pack 100 · A080H047A
|
0.8 µm · 47 mm · pack 100 · A080M047A
|
0.8 µm · 47 mm · pack 100 · A080R047A
|
0.8 µm · 47 mm · pack 100 · A080F47A
|
1 µm · 47 mm · pack 100
|
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Tamano de poro | 0.2 | 0.2 | 0.2 | 0.2 | 0.45 | 0.45 | 0.45 | 0.45 | 0.45 | 0.45 | 0.45 | 0.45 | 0.45 | 0.45 | 0.45 | 0.45 | 0.45 | 0.65 | 0.8 | 0.8 | 0.8 | 0.8 | 1 |
| Cuadricula | No | Yes | Yes | No | Yes | Yes | No | Yes | Yes | Yes | No | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
| Almohadilla | No | No | No | No | Yes | No | No | Yes | No | No | No | No | Yes | No | No | No | No | Yes | No | Yes | No | Yes | No |
| Tamano de paquete | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 1000 | 1000 | 1000 | 200 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| Diametro (mm) | 47 | 47 | 47 | 47 | 47 | 47 | 47 | 47 | 47 | 47 | 47 | 47 | 47 | 47 | 47 | 47 | 85 | 47 | 47 | 47 | 47 | 47 | 47 |
| Ensayo USP clase VI | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed | Passed |
| Union proteica BSA | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 | ~160 µg/cm2 |
| Extraibles | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% | <4% |
| Temperatura maxima de operacion | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) | 130 °C (266 °F) |
| Compatibilidad de sellado | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding | Ultrasonic, Heat, Radio Frequency, and Insert Molding |
| Espesor | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm | 150 µm |
| Rejilla | No | Yes | Yes | No | Yes | Yes | No | Yes | Yes | Yes | No | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
| Variante | Tamano de poro | Cuadricula | Almohadilla | Tamano de paquete | Diametro (mm) | Ensayo USP clase VI | Union proteica BSA | Extraibles | Temperatura maxima de operacion | Compatibilidad de sellado | Espesor | Rejilla |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
0.2 µm · 47 mm · pack 100 · A020G047A
|
0.2 | No | No | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | No |
|
0.2 µm · 47 mm · pack 100 · A020H047K
|
0.2 | Yes | No | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.2 µm · 47 mm · pack 100 · A020H047A
|
0.2 | Yes | No | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.2 µm · 47 mm · pack 100 · A020G047J
|
0.2 | No | No | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | No |
|
0.45 µm · 47 mm · pack 100 · A045F047A
|
0.45 | Yes | Yes | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.45 µm · 47 mm · pack 100 · A045H047A
|
0.45 | Yes | No | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.45 µm · 47 mm · pack 100 · A045G047A
|
0.45 | No | No | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | No |
|
0.45 µm · 47 mm · pack 100 · A045M047A
|
0.45 | Yes | Yes | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.45 µm · 47 mm · pack 100 · A045R047A
|
0.45 | Yes | No | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.45 µm · 47 mm · pack 100 · A045H047K
|
0.45 | Yes | No | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.45 µm · 47 mm · pack 100 · A045G047J
|
0.45 | No | No | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | No |
|
0.45 µm · 47 mm · pack 100 · A045W047A
|
0.45 | Yes | No | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.45 µm · 47 mm · pack 1000 · A045F047W
|
0.45 | Yes | Yes | 1000 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.45 µm · 47 mm · pack 1000 · A045H047W
|
0.45 | Yes | No | 1000 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.45 µm · 47 mm · pack 1000 · A045R047W
|
0.45 | Yes | No | 1000 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.45 µm · 47 mm · pack 200
|
0.45 | Yes | No | 200 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.45 µm · 85 mm · pack 100
|
0.45 | Yes | No | 100 | 85 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.65 µm · 47 mm · pack 100
|
0.65 | Yes | Yes | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.8 µm · 47 mm · pack 100 · A080H047A
|
0.8 | Yes | No | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.8 µm · 47 mm · pack 100 · A080M047A
|
0.8 | Yes | Yes | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.8 µm · 47 mm · pack 100 · A080R047A
|
0.8 | Yes | No | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
0.8 µm · 47 mm · pack 100 · A080F47A
|
0.8 | Yes | Yes | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |
|
1 µm · 47 mm · pack 100
|
1 | Yes | No | 100 | 47 | Passed | ~160 µg/cm2 | <4% | 130 °C (266 °F) | Ultrasonic, Heat, Radio Frequency, and Insert Molding | 150 µm | Yes |