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Filtration Cassettes - Tangential Flow

Filtration Cassettes - Tangential Flow

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Descripción general

Filtration Cassettes - Tangential Flow

Preguntas frecuentes

Vivaflow® Flujo cruzado devices offer a fast, cost-effective way to concentrate 0.1–5 L samples without large-scale systems. With PES or Hydrosart® membranes and single-use or reusable options, they can achieve up to 50× concentration—1 L in ~30 min or 5 L in <75 min—with near-total recovery after a single buffer rinse.

To reduce protein degradation, optimize buffer composition and minimize shear stress—e.g., use lower centrifuge speeds for linear proteins and devices with membrane areas suited to your starting volume. Vivaflow® Flujo cruzado devices minimize shear compared to centrifugal or Celda agitada systems, and diafiltration tools like the Vivaflow® reservoir help maintain optimal buffer conditions during concentration.

For virus concentration, use a MWCO ~⅓ of the target’s molecular Peso (or match Tamaño de poro to diameter). For Lentivirus (~90 nm), a 300 kDa Hydrosart® membrane offers the best balance of speed and recovery, with 100 kDa PES as a slower alternative. Always validate device, membrane, and MWCO for your specific virus.

To maximize protein concentration and recovery, match your membrane, MWCO, and device to your protein type. Consider sample properties, membrane material (PES, RC, CTA, HY), and choose a MWCO ~⅓ of target size. Optimize device design, pre-treat to reduce non-specific binding, and use dead-stop pockets or pre-filled filtrate tubes for maximum sample retrieval.

Especificación
VF-S050P0050-SV
VF05H0
VF05H1
VF05H2
VF05H4
VF20H0
VF20H1
VF20H2
VF20H4
VF20H9
VF20P0
VF20P1
VF20P2
VF20P3
VF20P4
VF20P7
VF20P9
Serie Vivaflow SU Vivaflow 50R Vivaflow 50R Vivaflow 50R Vivaflow 50R Vivaflow 200 Vivaflow 200 Vivaflow 200 Vivaflow 200 Vivaflow 200 Vivaflow 200 Vivaflow 200 Vivaflow 200 Vivaflow 200 Vivaflow 200 Vivaflow 200 Vivaflow 200
Membrana PES Hydrosart Hydrosart Hydrosart Hydrosart Hydrosart Hydrosart Hydrosart Hydrosart Hydrosart PES PES PES PES PES PES PES
Corte 50 kDa 10 kDa 5 kDa 30 kDa 100 kDa 10 kDa 5 kDa 30 kDa 100 kDa 2 kDa 10 kDa 5 kDa 30 kDa 50 kDa 100 kDa 0.2 um 3 kDa
Presentacion 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk